rabbit anti col 1  (Boster Bio)

Journal: Frontiers in Pharmacology

Article Title: Impact of baicalin and components of Scutellaria baicalensis on renal fibrosis of diabetic kidney disease

doi: 10.3389/fphar.2024.1480626

Figure Lengend Snippet: Effects on the expression of fibrotic protein in type 2 diabetic mice (A) Immunoblotting bands depicting collagen-II, collagen-I, and CTGF protein levels in mice in each group. (B) Expression of collagen-II protein in the mice of each group. (C) Expression of collagen-I protein in the mice of each group. (D) Expression of the CTGF protein in the mice of each group. (E) Relative expression of collagen-I mRNA in each group (n = 6, compared to the db/m group, # p < 0.05; compared to the db/db group, * p < 0.05). Abbreviations: HE: hematoxylin and eosin, PAS: periodic acid-Schiff, db/m: normal control group of non-diabetic mice, db/db: model of diabetes, BA: baicalin, MIX: multi-component mixture, Col- (I) collagen I, Col-II: collagen II, CTGF: connective tissue growth factor.

Article Snippet: The membranes were blocked in 5% (w/v) nonfat milk at room temperature for 1 h. They were then incubated overnight at 4°C with specific primary antibodies against TGF-β (25kDa, 1:1,000 dilution; #ab92486, Abcam, Cambridge, United Kingdom), Smad2/3 (60 kDa, 1:1,000 dilution; #12470, CST, Danvers, MA, United States), collagen I (Col-I) (220 kDa1:1,000 dilution, #72026 CST, Danvers, MA, United States), collagen II (Col-II) (200 kDa, 1:1,000 dilution, #43306S, CST, Danvers, MA, United States), and connective tissue growth factor (CTGF) (35 kDa, 1:1,000 dilution, #86641S, CST, Danvers, MA, United States).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Impact of baicalin and components of Scutellaria baicalensis on renal fibrosis of diabetic kidney disease

doi: 10.3389/fphar.2024.1480626

Figure Lengend Snippet: Effects indicating that baicalin and Scutellaria baicalensis mixture can inhibit the TGF-β/Smads signaling pathway in the renal tissues of HG mice (A) Immunoblotting bands depicting TGF-β, Smad2/3, and p- Smad2/3 protein levels in the mice from each group. (B) Expression levels of TGF-β protein in mice from each group. (C) Expression levels of p-Smad2/3 protein in mice from each group. (D) Expression levels of Smad2/3 protein in the mice from each group. (E) Photomicrographs of Smad2/3 and TGF-β staining of the kidney sections from each group, observed under a light microscope (×400). (F) Quantitative statistical plot of Smad2/3 in kidney sections. (G) Quantitative statistical plot of TGF-β in kidney sections (n = 6, compared to the db/m group, # p < 0.05; compared to the db/db group, * p < 0.05). Abbreviations: HE: hematoxylin and eosin, PAS: periodic acid-Schiff, db/m: normal control group of non-diabetic mice, db/db: model of diabetes, BA: baicalin, MIX: multi-component mixture, Col- (I) collagen, I Col-II: collagen II, TGF-β: transforming growth factor-β.

Article Snippet: The membranes were blocked in 5% (w/v) nonfat milk at room temperature for 1 h. They were then incubated overnight at 4°C with specific primary antibodies against TGF-β (25kDa, 1:1,000 dilution; #ab92486, Abcam, Cambridge, United Kingdom), Smad2/3 (60 kDa, 1:1,000 dilution; #12470, CST, Danvers, MA, United States), collagen I (Col-I) (220 kDa1:1,000 dilution, #72026 CST, Danvers, MA, United States), collagen II (Col-II) (200 kDa, 1:1,000 dilution, #43306S, CST, Danvers, MA, United States), and connective tissue growth factor (CTGF) (35 kDa, 1:1,000 dilution, #86641S, CST, Danvers, MA, United States).

Techniques: Western Blot, Expressing, Staining, Light Microscopy, Control